Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus.
Stem Cell Res
; 53: 102310, 2021 05.
Article
em En
| MEDLINE
| ID: mdl-33812335
ABSTRACT
MERTK mutations are associate with rod-cone dystrophies. To enable investigations into the mechanism of this disease, we generated a cell line resource of H9 human embryonic stem cells harboring large fragment deletion mutation in a homozygous state in exon 19 of the MERTK gene. This subline expressed pluripotent stem cell markers, presented a normal karyotype, and preserved the ability to differentiate into endodermal, mesodermal, and ectodermal lineages.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Embrionárias Humanas
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article