Development of a chromatography-free method for high-throughput MS-based bioanalysis of therapeutic monoclonal antibodies.
Bioanalysis
; 13(9): 725-735, 2021 May.
Article
em En
| MEDLINE
| ID: mdl-33856232
ABSTRACT
Aim:
Our objective was to test the feasibility of developing an LC-free, MS-based approach for high-throughput bioanalysis of humanized therapeutic monoclonal antibodies.Methodology:
A universal tryptic peptide from human IgG1, IgG3 and IgG4 was selected as the surrogate peptide for quantitation. After tryptic digestion, the surrogate peptide was fractionated via solid-phase extraction before being subjected to direct infusion-based MS/MS analysis. A high-resolution, multiplexed (MSX = 2) parallel reaction monitoring method was developed for data acquisition. Results &conclusion:
This proof-of-concept study demonstrated the feasibility of achieving high-throughput MS-based bioanalysis of monoclonal antibodies using an LC-free workflow with sensitivity comparable to conventional LC-MS/MS-based methods.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ensaios de Triagem em Larga Escala
/
Desenvolvimento de Medicamentos
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article