Biomimetic Molecular Clamp Nanopores for Simultaneous Quantifications of NAD+ and NADH.
Anal Chem
; 93(18): 7118-7124, 2021 05 11.
Article
em En
| MEDLINE
| ID: mdl-33905222
ABSTRACT
NADH/NAD+ is pivotal to fundamental biochemistry research and molecular diagnosis, but recognition and detection for them are a big challenge at the single-molecule level. Inspired by the biological system, here, we designed and synthesized a biomimetic NAD+/NADH molecular clamp (MC), octakis-(6-amino-6-deoxy)-γ-cyclomaltooctaose, and harbored in the engineered α-HL(M113R)7 nanopore, forming a novel single-molecule biosensor. The single-molecule measurement possesses high selectivity and a high signal-to-noise ratio, allowing to simultaneously recognize and detect for sensing NADH/NAD+ and their transformations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Nanoporos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article