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CRISPR-Cas tools to study gene function in cytokinesis.
Husser, Mathieu C; Skaik, Noha; Martin, Vincent J J; Piekny, Alisa.
Afiliação
  • Husser MC; Biology Department, Concordia University, Montréal, QC, H4B 1R6, Canada.
  • Skaik N; Biology Department, Concordia University, Montréal, QC, H4B 1R6, Canada.
  • Martin VJJ; Biology Department, Concordia University, Montréal, QC, H4B 1R6, Canada.
  • Piekny A; Center for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B 1R6, Canada.
J Cell Sci ; 134(8)2021 04 15.
Article em En | MEDLINE | ID: mdl-33912919
Cytokinesis is the process that separates a cell into two daughter cells at the end of mitosis. Most of our knowledge of cytokinesis comes from overexpression studies, which affects our interpretation of protein function. Gene editing can circumvent this issue by introducing functional mutations or fluorescent probes directly into a gene locus. However, despite its potential, gene editing is just starting to be used in the field of cytokinesis. Here, we discuss the benefits of using gene editing tools for the study of cytokinesis and highlight recent studies that successfully used CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) technology to answer critical questions regarding the function of cytokinesis proteins. We also present methodologies for editing essential genes and discuss how CRISPR interference (CRISPRi) and activation (CRISPRa) can enable precise control of gene expression to answer important questions in the field. Finally, we address the need for gene editing to study cytokinesis in more physiologically relevant contexts. Therefore, this Review provides a roadmap for gene editing to be used in the study of cytokinesis and other cellular processes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas / Sistemas CRISPR-Cas Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas / Sistemas CRISPR-Cas Idioma: En Ano de publicação: 2021 Tipo de documento: Article