Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units.
Mar Drugs
; 19(4)2021 Apr 16.
Article
em En
| MEDLINE
| ID: mdl-33923695
ABSTRACT
Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine the application on the improvement of hygiene problems caused by biofilm infection of Pseudomonas aeruginosa in dental units. Pseudomonas bacteria were isolated from dental chair units and used to grow static biofilms in the laboratory. A water filter system was designed to test the antifouling activity of YtnP in Laboratory, to simulate the biofilm contamination on water filter in dental unit water lines. The results demonstrated that the enzyme of YtnP was able to degrade the N-acyl homoserine lactones, significantly inhibited the EPS generation, biofilm formation, and virulence factors production (pyocyanin and rhamnolipid) of P. aeruginosa, and was efficient on the antifouling against P. aeruginosa. The findings in this study indicated the possibility of YtnP as novel disinfectant reagent for hygiene treatment in dental units.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
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Bacillus
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Proteínas de Bactérias
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Microbiologia da Água
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Abastecimento de Água
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Hidrolases de Éster Carboxílico
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Descontaminação
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Purificação da Água
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Instalações Odontológicas
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Percepção de Quorum
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article