Detection of monoclonal protein by capillary zone electrophoresis can be challenged by iodinated contrast agent interference: a case report.
Biochem Med (Zagreb)
; 31(2): 021001, 2021 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-33927559
ABSTRACT
The detection of monoclonal immunoglobulins is a key element in the diagnosis of monoclonal gammopathy. In clinical practice, screening and measurement of monoclonal proteins are commonly performed using capillary zone electrophoresis (CZE). Some exogenous substances, such as iodinated contrast agents, absorb incident UV light at the same wavelengths as the peptide bonds and may therefore interfere with the detection of proteins in CZE. We herein use the description of a case to illustrate that iodinated contrast agents can mask the presence of monoclonal immunoglobulins in CZE and we discuss the strategy needed to confirm this interference. Performing immunofixation, immunosubtraction, or a second CZE at a distance from the first blood sample is not only necessary to confirm the presence of an iodinated contrast media interference but also to ensure the absence of monoclonal proteins.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paraproteinemias
/
Imunoglobulinas
/
Eletroforese Capilar
/
Meios de Contraste
Tipo de estudo:
Diagnostic_studies
Limite:
Aged80
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article