Studying Neuronal Biology Using Spinning Disc Confocal Microscopy.
Methods Mol Biol
; 2304: 265-283, 2021.
Article
em En
| MEDLINE
| ID: mdl-34028722
ABSTRACT
Cytoskeletal integrity is essential for neuronal complexity and functionality. Certain inherited neurological diseases are associated with mutated genes that directly or indirectly compromise cytoskeletal stability. While the large size and complexity of the neurons grown in culture poses certain challenges for imaging, live-cell imaging is an excellent approach to determine the morphological consequences of such mutants. This protocol details the use of spinning disk confocal microscopy and image analysis tools to evaluate branching and neurite length of healthy iPSC-derived glutamatergic neurons that express specific fluorescent proteins. The protocols can be adapted to neuronal cell lines of choice by the investigator.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Microscopia Confocal
/
Células-Tronco Pluripotentes Induzidas
/
Mutação
/
Neurônios
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article