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DMA-tudor interaction modules control the specificity of in vivo condensates.
Courchaine, Edward M; Barentine, Andrew E S; Straube, Korinna; Lee, Dong-Ryoung; Bewersdorf, Joerg; Neugebauer, Karla M.
Afiliação
  • Courchaine EM; Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
  • Barentine AES; Cell Biology, Yale University, New Haven, CT 06520, USA; Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
  • Straube K; Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
  • Lee DR; Cell Biology, Yale University, New Haven, CT 06520, USA.
  • Bewersdorf J; Cell Biology, Yale University, New Haven, CT 06520, USA; Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
  • Neugebauer KM; Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA; Cell Biology, Yale University, New Haven, CT 06520, USA. Electronic address: karla.neugebauer@yale.edu.
Cell ; 184(14): 3612-3625.e17, 2021 07 08.
Article em En | MEDLINE | ID: mdl-34115980
Biomolecular condensation is a widespread mechanism of cellular compartmentalization. Because the "survival of motor neuron protein" (SMN) is implicated in the formation of three different membraneless organelles (MLOs), we hypothesized that SMN promotes condensation. Unexpectedly, we found that SMN's globular tudor domain was sufficient for dimerization-induced condensation in vivo, whereas its two intrinsically disordered regions (IDRs) were not. Binding to dimethylarginine (DMA) modified protein ligands was required for condensate formation by the tudor domains in SMN and at least seven other fly and human proteins. Remarkably, asymmetric versus symmetric DMA determined whether two distinct nuclear MLOs-gems and Cajal bodies-were separate or "docked" to one another. This substructure depended on the presence of either asymmetric or symmetric DMA as visualized with sub-diffraction microscopy. Thus, DMA-tudor interaction modules-combinations of tudor domains bound to their DMA ligand(s)-represent versatile yet specific regulators of MLO assembly, composition, and morphology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arginina / Proteínas do Complexo SMN / Condensados Biomoleculares Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arginina / Proteínas do Complexo SMN / Condensados Biomoleculares Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article