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Effects of pore size and porosity on cytocompatibility and osteogenic differentiation of porous titanium.
Yao, Yi-Tong; Yang, Yue; Ye, Qi; Cao, Shan-Shan; Zhang, Xin-Ping; Zhao, Ke; Jian, Yutao.
Afiliação
  • Yao YT; Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
  • Yang Y; Department of Stomatology, Shenzhen People's Hospital (Second Clinical Medical School of Jinan University; First Affiliated Hospital of Southern University of Science and Technology), Shenzhen, China.
  • Ye Q; Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, China.
  • Cao SS; School of Materials Science and Engineering, South China University of Technology, Guangzhou, China.
  • Zhang XP; School of Materials Science and Engineering, South China University of Technology, Guangzhou, China. mexzhang@scut.edu.cn.
  • Zhao K; Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China. zhaoke@mail.sysu.edu.cn.
  • Jian Y; Institute of Stomatological Research, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China. jianyt@mail.sysu.edu.cn.
J Mater Sci Mater Med ; 32(6): 72, 2021 Jun 14.
Article em En | MEDLINE | ID: mdl-34125310
To find out the optimal porosity and pore size of porous titanium (Ti) regarding the cytocompatibility and osteogenic differentiation. Six groups of porous Ti samples with different porosities and pore sizes were fabricated by the powder metallurgy process. The microstructure and compressive mechanical properties were characterized. The cytocompatibility was examined by a series of biological tests as protein absorption with BCA assay kit, cell attachment with laser scanning confocal microscopy and vinculin expression, cell proliferation with CCK-8 assay. Cell differentiation and calcification were detected by qPCR and Alizarin Red S dying respectively. Pores distributed homogeneously throughout the porous Ti samples. The compressive test results showed that Young's modulus ranged from 2.80 ± 0.03 GPa to 5.43 ± 0.34 GPa and the compressive strength increased from 112.4 ± 3.6 MPa to 231.1 ± 9.4 MPa. Porous Ti with high porosity (53.3 ± 1.2%) and small pore size (191.6 ± 3.7 µm) adsorbed more proteins. More MC3T3-E1 cells adhered onto dense Ti samples than onto any other porous ones already after culture and no difference was identified within the porous groups. The porous structure of porous Ti with a porosity of 53.3 ± 1.2% and an average pore size of 191.6 ± 3.7 µm facilitated cell differentiation and calcification. Small pores were not beneficial to the osteo-initiation at the very beginning. Porous Ti with a porosity of 53.3 ± 1.2% and an average pore size of 191.6 ± 3.7 µm fabricated by powder metallurgy process showed the expected mechanical property and improved osseointegration as implants in dental treatment.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Titânio / Teste de Materiais / Força Compressiva Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Titânio / Teste de Materiais / Força Compressiva Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article