Selective 13 C-Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins.
Angew Chem Int Ed Engl
; 60(34): 18777-18782, 2021 08 16.
Article
em En
| MEDLINE
| ID: mdl-34128568
ABSTRACT
A combined chemo-enzymatic synthesis/NMR-based methodology is presented to identify, in unambiguous manner, the distinctive binding epitope within repeating sugar oligomers when binding to protein receptors. The concept is based on the incorporation of 13 C-labels at specific monosaccharide units, selected within a repeating glycan oligomeric structure. No new chemical tags are added, and thus the chemical entity remains the same, while the presence of the 13 C-labeled monosaccharide breaks the NMR chemical shift degeneracy that occurs in the non-labeled compound and allows the unique identification of the different components of the oligomer. The approach is demonstrated by a proof-of-concept study dealing with the interaction of a polylactosamine hexasaccharide with five different galectins that display distinct preferences for these entities.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Ressonância Magnética Nuclear Biomolecular
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Galectinas
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Amino Açúcares
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Epitopos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article