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Downstream of GA4, PbCYP78A6 participates in regulating cell cycle-related genes and parthenogenesis in pear (Pyrus bretshneideri Rehd.).
Zhang, Haiqi; Han, Wei; Wang, Huibin; Cong, Liu; Zhai, Rui; Yang, Chengquan; Wang, Zhigang; Xu, Lingfei.
Afiliação
  • Zhang H; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China.
  • Han W; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China.
  • Wang H; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China.
  • Cong L; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China.
  • Zhai R; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China.
  • Yang C; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China.
  • Wang Z; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China.
  • Xu L; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Shaanxi Province, Taicheng Road No.3, Yangling, 712100, China. lingfxu2013@sina.com.
BMC Plant Biol ; 21(1): 292, 2021 Jun 24.
Article em En | MEDLINE | ID: mdl-34167472
BACKGROUND: Parthenocarpy results in traits attractive to both consumers and breeders, and it overcomes the obstacle of self-incompatibility in the fruit set of horticultural crops, including pear (Pyrus bretshneider). However, there is limited knowledge regarding the genetic and molecular mechanisms that regulate parthenogenesis. RESULTS: Here, in a transcriptional comparison between pollination-dependent fruit and GA4-induced parthenocarpy, PbCYP78A6 was identified and proposed as a candidate gene involved in parthenocarpy. PbCYP78A6 is similar to Arabidopsis thaliana CYP78A6 and highly expressed in pear hypanthia. The increased PbCYP78A6 expression, as assessed by RT-qPCR, was induced by pollination and GA4 exposure. The ectopic overexpression of PbCYP78A6 contributed to parthenocarpic fruit production in tomato. The PbCYP78A6 expression coincided with fertilized and parthenocarpic fruitlets development and the expression of fruit development-related genes as assessed by cytological observations and RT-qPCR, respectively. PbCYP78A6 RNA interference and overexpression in pear calli revealed that the gene is an upstream regulator of specific fruit development-related genes in pear. CONCLUSIONS: Our findings indicate that PbCYP78A6 plays a critical role in fruit formation and provide insights into controlling parthenocarpy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Partenogênese / Proteínas de Plantas / Ciclo Celular / Genes de Plantas / Sistema Enzimático do Citocromo P-450 / Pyrus Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Partenogênese / Proteínas de Plantas / Ciclo Celular / Genes de Plantas / Sistema Enzimático do Citocromo P-450 / Pyrus Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article