Comparison between high-frequency irreversible electroporation and irreversible electroporation ablation of small swine liver: follow-up of DCE-MRI and pathological observations.
Chin Med J (Engl)
; 134(17): 2081-2090, 2021 Jun 24.
Article
em En
| MEDLINE
| ID: mdl-34172620
ABSTRACT
BACKGROUND:
High-frequency irreversible electroporation (H-FIRE) is a novel, next-generation nanoknife technology with the advantage of relieving irreversible electroporation (IRE)-induced muscle contractions. However, the difference between IRE and H-FIRE with distinct ablation parameters was not clearly defined. This study aimed to compare the efficacy of the two treatments in vivo.METHODS:
Ten Bama miniature swine were divided into two group five in the 1-day group and five in the 7-day group. The efficacy of IRE and H-FIRE ablation was compared by volume transfer constant (Krans), rate constant (Kep) and extravascular extracellular volume fraction (Ve) value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), size of the ablation zone, and histologic analysis. Each animal underwent the IRE and H-FIRE. Temperatures of the electrodes were measured during ablation. DCE-MRI images were obtained 1, 4, and 7 days after ablation in the 7-day group. All animals in the two groups were euthanized 1 day or 7 days after ablation, and subsequently, IRE and H-FIRE treated liver tissues were collected for histological examination. Student's t test or Mann-Whitney U test was applied for comparing any two groups. One-way analysis of variance (ANOVA) test and Welch's ANOVA test followed by Holm-Sidak's multiple comparisons test, one-way ANOVA with repeated measures followed by Bonferroni test, or Kruskal-Wallis H test followed by Dunn's multiple comparison test was used for multiple group comparisons and post hoc analyses. Pearson correlation coefficient test was conducted to analyze the relationship between two variables.RESULTS:
Higher Ve was seen in IRE zone than in H-FIRE zone (0.14â±â0.02 vs. 0.08â±â0.05, tâ=â2.408, Pâ=â0.043) on day 4, but no significant difference was seen in Ktrans or Kep between IRE and H-FIRE zones at all time points (all Pâ>â0.05). For IRE zone, the greatest Ktrans was seen on day 7, which was significantly higher than that on day 1 (Pâ=â0.033). The ablation zone size of H-FIRE was significantly larger than IRE 1 day (4.74â±â0.88âcm2vs. 3.20â±â0.77âcm2, tâ=â3.241, Pâ=â0.009) and 4 days (2.22â±â0.83âcm2vs. 1.30â±â0.50âcm2, tâ=â2.343, Pâ=â0.041) after treatment. Apoptotic index (0.05â±â0.02 vs. 0.73â±â0.06 vs. 0.68â±â0.07, Fâ=â241.300, Pâ<â0.001) and heat shock protein 70 (HSP70) (0.03â±â0.01 vs. 0.46â±â0.09 vs. and 0.42â±â0.07, Fâ=â64.490, Pâ<â0.001) were significantly different between the untreated, IRE and H-FIRE zones, but no significant difference was seen in apoptotic index or HSP70 between IRE and H-FIRE zone (both Pâ>â0.05). Electrode temperature variations were not significantly different between the two zones (18.00â±â3.77°C vs. 16.20â±â7.45°C, tâ=â0.682, Pâ=â0.504). The Ktrans value (râ=â0.940, Pâ=â0.017) and the Kep value (râ=â0.895, Pâ=â0.040) of the H-FIRE zone were positively correlated with the number of hepatocytes in the ablation zone.CONCLUSIONS:
H-FIRE showed a comparable ablation effect to IRE. DCE-MRI has the potential to monitor the changes of H-FIRE ablation zone.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imageamento por Ressonância Magnética
/
Eletroporação
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article