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Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp14 RNA cap methyltransferase.
Basu, Souradeep; Mak, Tiffany; Ulferts, Rachel; Wu, Mary; Deegan, Tom; Fujisawa, Ryo; Tan, Kang Wei; Lim, Chew Theng; Basier, Clovis; Canal, Berta; Curran, Joseph F; Drury, Lucy S; McClure, Allison W; Roberts, Emma L; Weissmann, Florian; Zeisner, Theresa U; Beale, Rupert; Cowling, Victoria H; Howell, Michael; Labib, Karim; Diffley, John F X.
Afiliação
  • Basu S; Cell Cycle Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Mak T; Cell Cycle Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Ulferts R; Cell Biology of Infection Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Wu M; High Throughput Screening, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Deegan T; The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
  • Fujisawa R; The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
  • Tan KW; Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Lim CT; Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Basier C; Cell Cycle Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Canal B; Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Curran JF; Cell Cycle Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Drury LS; Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • McClure AW; Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Roberts EL; Cell Cycle Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Weissmann F; Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Zeisner TU; Cell Cycle Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Beale R; Cell Biology of Infection Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Cowling VH; Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
  • Howell M; High Throughput Screening, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
  • Labib K; The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.
  • Diffley JFX; Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K.
Biochem J ; 478(13): 2481-2497, 2021 07 16.
Article em En | MEDLINE | ID: mdl-34198328
The COVID-19 pandemic has presented itself as one of the most critical public health challenges of the century, with SARS-CoV-2 being the third member of the Coronaviridae family to cause a fatal disease in humans. There is currently only one antiviral compound, remdesivir, that can be used for the treatment of COVID-19. To identify additional potential therapeutics, we investigated the enzymatic proteins encoded in the SARS-CoV-2 genome. In this study, we focussed on the viral RNA cap methyltransferases, which play key roles in enabling viral protein translation and facilitating viral escape from the immune system. We expressed and purified both the guanine-N7 methyltransferase nsp14, and the nsp16 2'-O-methyltransferase with its activating cofactor, nsp10. We performed an in vitro high-throughput screen for inhibitors of nsp14 using a custom compound library of over 5000 pharmaceutical compounds that have previously been characterised in either clinical or basic research. We identified four compounds as potential inhibitors of nsp14, all of which also showed antiviral capacity in a cell-based model of SARS-CoV-2 infection. Three of the four compounds also exhibited synergistic effects on viral replication with remdesivir.
Assuntos
Antivirais/farmacologia; Avaliação Pré-Clínica de Medicamentos; Exorribonucleases/antagonistas & inibidores; Metiltransferases/antagonistas & inibidores; Capuzes de RNA/metabolismo; SARS-CoV-2/enzimologia; Bibliotecas de Moléculas Pequenas/farmacologia; Proteínas não Estruturais Virais/antagonistas & inibidores; Monofosfato de Adenosina/análogos & derivados; Monofosfato de Adenosina/farmacologia; Alanina/análogos & derivados; Alanina/farmacologia; Animais; Antivirais/química; Clorobenzenos/farmacologia; Chlorocebus aethiops; Ensaios Enzimáticos; Exorribonucleases/genética; Exorribonucleases/isolamento & purificação; Exorribonucleases/metabolismo; Transferência Ressonante de Energia de Fluorescência; Ensaios de Triagem em Larga Escala; Indazóis/farmacologia; Indenos/farmacologia; Indóis/farmacologia; Metiltransferases/genética; Metiltransferases/isolamento & purificação; Metiltransferases/metabolismo; Nitrilas/farmacologia; Fenotiazinas/farmacologia; Purinas/farmacologia; Reprodutibilidade dos Testes; SARS-CoV-2/efeitos dos fármacos; Bibliotecas de Moléculas Pequenas/química; Especificidade por Substrato; Trifluperidol/farmacologia; Células Vero; Proteínas não Estruturais Virais/genética; Proteínas não Estruturais Virais/isolamento & purificação; Proteínas não Estruturais Virais/metabolismo; Proteínas Virais Reguladoras e Acessórias/genética; Proteínas Virais Reguladoras e Acessórias/isolamento & purificação; Proteínas Virais Reguladoras e Acessórias/metabolismo
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Capuzes de RNA / Proteínas não Estruturais Virais / Avaliação Pré-Clínica de Medicamentos / Exorribonucleases / Bibliotecas de Moléculas Pequenas / SARS-CoV-2 / Metiltransferases Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Capuzes de RNA / Proteínas não Estruturais Virais / Avaliação Pré-Clínica de Medicamentos / Exorribonucleases / Bibliotecas de Moléculas Pequenas / SARS-CoV-2 / Metiltransferases Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article