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Crosstalk between Macrophages and Myxoid Liposarcoma Cells Increases Spreading and Invasiveness of Tumor Cells.
Minopoli, Michele; Sarno, Sabrina; Cannella, Lucia; Tafuto, Salvatore; Scognamiglio, Gosuè; Gallo, Michele; Fazioli, Flavio; Azzaro, Rosa; Apice, Gaetano; De Angelis, Biagio; Tamborini, Elena; Garofalo, Cecilia; Pignochino, Ymera; Mercatali, Laura; Ibrahim, Toni; Falcioni, Rita; Valenti, Beatrice; Maestro, Roberta; Scotlandi, Katia; De Chiara, Annarosaria; Carriero, Maria Vincenza.
Afiliação
  • Minopoli M; Neoplastic Progression Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Sarno S; Neoplastic Progression Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Cannella L; Medical Oncology Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Tafuto S; Medical Oncology Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Scognamiglio G; Pathology Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Gallo M; Musculoskeletal Surgery Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Fazioli F; Musculoskeletal Surgery Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Azzaro R; Transfusion Medicine Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • Apice G; Medical Oncology Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
  • De Angelis B; Department of Onco-Haematology and Cell and Gene Therapy Unit, Bambino Gesù Children's Hospital, IRCCS, 00165 Rome, Italy.
  • Tamborini E; Department of Pathology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy.
  • Garofalo C; Advanced Translational Research Laboratory, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy.
  • Pignochino Y; Department of Clinical and Biological Sciences, University of Turin, 10124 Torino, Italy.
  • Mercatali L; Division of Medical Oncology, Candiolo Cancer Institute, FPO-IRCCS, 10060 Candiolo, Italy.
  • Ibrahim T; Osteoncology and Rare Tumors Center, Istituto Romagnolo per lo Studio dei Tumori "Dino Amadori" (IRST) IRCCS, 47010 Meldola, Italy.
  • Falcioni R; Osteoncology and Rare Tumors Center, Istituto Romagnolo per lo Studio dei Tumori "Dino Amadori" (IRST) IRCCS, 47010 Meldola, Italy.
  • Valenti B; Department of Research, Diagnosis and Innovative Technology, IRCCS Regina Elena National Cancer Institute, 00128 Rome, Italy.
  • Maestro R; Oncogenetics and Functional Oncogenomics, Centro di Riferimento Oncologico di Aviano (CRO Aviano) IRCCS, National Cancer Institute, 33081 Aviano, Italy.
  • Scotlandi K; Oncogenetics and Functional Oncogenomics, Centro di Riferimento Oncologico di Aviano (CRO Aviano) IRCCS, National Cancer Institute, 33081 Aviano, Italy.
  • De Chiara A; Laboratory of Experimental Oncology, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
  • Carriero MV; Pathology Unit, Istituto Nazionale Tumori IRCCS 'Fondazione G. Pascale', 80131 Naples, Italy.
Cancers (Basel) ; 13(13)2021 Jun 30.
Article em En | MEDLINE | ID: mdl-34209309
Myxoid liposarcoma (MLPS) is the second most common subtype of liposarcoma and has tendency to metastasize to soft tissues. To date, the mechanisms of invasion and metastasis of MLPS remain unclear, and new therapeutic strategies that improve patients' outcomes are expected. In this study, we analyzed by immunohistochemistry the immune cellular components and microvessel density in tumor tissues from patients affected by MLPS. In order to evaluate the effects of primary human MLPS cells on macrophage polarization and, in turn, the ability of macrophages to influence invasiveness of MLPS cells, non-contact and 3D organotypic co-cultures were set up. High grade MLPS tissues were found heavily vascularized, exhibited a CD3, CD4, and CD8 positive T lymphocyte-poor phenotype and were massively infiltrated by CD163 positive M2-like macrophages. Conversely, low grade MLPS tissues were infiltrated by a discrete amount of CD3, CD4, and CD8 positive T lymphocytes and a scarce amount of CD163 positive macrophages. Kaplan-Meier analysis revealed a shorter Progression Free Survival in MLPS patients whose tumor tissues were highly vascularized and heavily infiltrated by CD163 positive macrophages, indicating a clear-cut link between M2-like macrophage abundance and poor prognosis in patients. Moreover, we documented that, in co-culture, soluble factors produced by primary human MLPS cells induce macrophage polarization toward an M2-like phenotype which, in turn, increases MLPS cell capability to spread into extracellular matrix and to cross endothelial monolayers. The identification of M2-like polarization factors secreted by MLPS cells may allow to develop novel targeted therapies counteracting MLPS progression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article