Robust and recyclable magnetic nanobiocatalysts for extraction of anthocyanin from black rice.
Food Chem
; 364: 130447, 2021 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-34214946
ABSTRACT
Anthocyanins, which are natural pigments and nutraceuticals, can be extracted from plant materials using enzyme-assisted methods. However, the enzymes used are often expensive, fragile, and hard to recover/reuse. In this study, cellulase and α-amylase were immobilized on amino-functionalized magnetic nanoparticles to prepare a magnetic nanobiocatalyst. The enzymes in this nanobiocatalyst exhibited higher stability and greater catalytic activity than free enzymes, including good thermal stability (50 to 70â) and pH stability (pH 4.5-7.5). Nanobiocatalyst efficacy was demonstrated by extracting anthocyanins from black rice, with a maximum yield of 266 mg anthocyanin/100 g black rice obtained. After six reuse cycles, cellulase and α-amylase retained around 70% and 64% of their activity, respectively. Immobilization also increased their reusability. In summary, a novel magnetic nanobiocatalyst was developed for extracting anthocyanins from black rice, which may also have other applications within the food industry.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Celulase
/
Antocianinas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article