A protocol for human bone marrow adipocyte isolation and purification.
STAR Protoc
; 2(3): 100629, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34235494
Primary human bone marrow adipocytes (BM-Ads) display a specific metabolism that is not recapitulated by in vitro differentiated bone marrow mesenchymal stromal cells. These findings highlight the need for using primary BM-Ads in studies of the metabolic impact of BM-Ads on surrounding cells. Here, we present a protocol for isolating human BM-Ads from bone marrow aspirates and verifying adipocyte suspension purity. These isolated and purified BM-Ads can be used for functional assays or frozen for molecular analyses. For complete details on the use and execution of this protocol, please refer to Attane et al. (2020).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Adipócitos
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article