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Integrated Multi-Omics Analysis of Mechanisms Underlying Yeast Ethanol Tolerance.
Sostaric, Nikolina; Arslan, Ahmed; Carvalho, Bernardo; Plech, Marcin; Voordeckers, Karin; Verstrepen, Kevin J; van Noort, Vera.
Afiliação
  • Sostaric N; Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
  • Arslan A; Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
  • Carvalho B; Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
  • Plech M; Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
  • Voordeckers K; VIB-KU Leuven Center for Microbiology, Bioincubator, Gaston Geenslaan 1, B-3001 Leuven, Belgium.
  • Verstrepen KJ; Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
  • van Noort V; VIB-KU Leuven Center for Microbiology, Bioincubator, Gaston Geenslaan 1, B-3001 Leuven, Belgium.
J Proteome Res ; 20(8): 3840-3852, 2021 08 06.
Article em En | MEDLINE | ID: mdl-34236875
ABSTRACT
For yeast cells, tolerance to high levels of ethanol is vital both in their natural environment and in industrially relevant conditions. We recently genotyped experimentally evolved yeast strains adapted to high levels of ethanol and identified mutations linked to ethanol tolerance. In this study, by integrating genomic sequencing data with quantitative proteomics profiles from six evolved strains (data set identifier PXD006631) and construction of protein interaction networks, we elucidate exactly how the genotype and phenotype are related at the molecular level. Our multi-omics approach points to the rewiring of numerous metabolic pathways affected by genomic and proteomic level changes, from energy-producing and lipid pathways to differential regulation of transposons and proteins involved in cell cycle progression. One of the key differences is found in the energy-producing metabolism, where the ancestral yeast strain responds to ethanol by switching to respiration and employing the mitochondrial electron transport chain. In contrast, the ethanol-adapted strains appear to have returned back to energy production mainly via glycolysis and ethanol fermentation, as supported by genomic and proteomic level changes. This work is relevant for synthetic biology where systems need to function under stressful conditions, as well as for industry and in cancer biology, where it is important to understand how the genotype relates to the phenotype.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteômica Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteômica Idioma: En Ano de publicação: 2021 Tipo de documento: Article