Your browser doesn't support javascript.
loading
Enhancing In Vitro Stability of Albumin Microbubbles Produced Using Microfluidic T-Junction Device.
Khan, Aaqib H; Surwase, Swarupkumar; Jiang, Xinyue; Edirisinghe, Mohan; Dalvi, Sameer V.
Afiliação
  • Khan AH; Chemical Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382355, Gujarat India.
  • Surwase S; Chemical Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382355, Gujarat India.
  • Jiang X; Department of Mechanical Engineering, University College London (UCL), London WC1E 7JE, United Kingdom.
  • Edirisinghe M; Department of Mechanical Engineering, University College London (UCL), London WC1E 7JE, United Kingdom.
  • Dalvi SV; Chemical Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382355, Gujarat India.
Langmuir ; 38(17): 5052-5062, 2022 05 03.
Article em En | MEDLINE | ID: mdl-34264681
Microfluidics is an efficient technique for continuous synthesis of monodispersed microbubbles. However, microbubbles produced using microfluidic devices possess lower stability due to quick dissolution of core gas when exposed to an aqueous environment. This work aims at generating highly stable monodispersed albumin microbubbles using microfluidic T-junction devices. Microbubble generation was facilitated by an aqueous phase consisting of bovine serum albumin (BSA) as a model protein and nitrogen (N2) gas. Microbubbles were chemically cross-linked using dilute glutaraldehyde (0.75% v/v) solution and thermally cross-linked by collecting microbubbles in hot water maintained at 368 (±2) K. These microbubbles were then subjected to in vitro dissolution in an air-saturated water. Microbubbles cross-linked with a combined treatment of thermal and chemical cross-linking (TC & CC) had longer dissolution time compared to microbubbles chemically cross-linked (CC) alone, thermally cross-linked (TC) alone, and non-cross-linked microbubbles. Circular dichroism (CD) spectroscopy analysis revealed that percent reduction in alpha-helices of BSA was higher for the combined treatment of TC & CC when compared to other treatments. In contrast to non-cross-linked microbubbles where microbubble shell dissolved completely, a significant shell detachment was observed during the final phase of the dissolution for cross-linked microbubbles captured using high speed camera, depending upon the extent of cross-linking of the microbubble shell. SEM micrographs of the microbubble shell revealed the shell thickness of microbubbles treated with TC & CC to be highest compared to only thermally or only chemically cross-linked microbubbles. Comparison of microbubble dissolution data to a mass transfer model showed that shell resistance to gas permeation was highest for microbubbles subjected to a combined treatment of TC & CC.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microfluídica / Microbolhas Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microfluídica / Microbolhas Idioma: En Ano de publicação: 2022 Tipo de documento: Article