Purification of active human vacuolar H<sup>+</sup>-ATPase in native lipid-containing nanodiscs.

Oot, Rebecca A; Yao, Yeqi; Manolson, Morris F; Wilkens, Stephan

Purification of active human vacuolar H⁺-ATPase in native lipid-containing nanodiscs.

Oot, Rebecca A; Yao, Yeqi; Manolson, Morris F; Wilkens, Stephan.

Afiliação

Oot RA; Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, USA.
Yao Y; Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Manolson MF; Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Wilkens S; Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, USA. Electronic address: wilkenss@upstate.edu.

J Biol Chem ; 297(2): 100964, 2021 08.

Article em En | MEDLINE | ID: mdl-34270960

Assuntos

ATPases Vacuolares Próton-Translocadoras; Transporte Biológico; Membrana Celular/metabolismo; Humanos; Saccharomyces cerevisiae/metabolismo

Palavras-chave

ATP6V0a4; MS; human; membrane protein; native lipid nanodisc; protein purification; vacuolar H(+)-ATPase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: ATPases Vacuolares Próton-Translocadoras Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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