CryoEM reconstructions of membrane proteins solved in several amphipathic solvents, nanodisc, amphipol and detergents, yield amphipathic belts of similar sizes corresponding to a common ordered solvent layer.

Zampieri, Veronica; Gobet, Alexia; Robert, Xavier; Falson, Pierre; Chaptal, Vincent

Zampieri, Veronica; Gobet, Alexia; Robert, Xavier; Falson, Pierre; Chaptal, Vincent.

Afiliação

Zampieri V; Molecular Microbiology and Structural Biochemistry Laboratory (CNRS UMR 5086), University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France.
Gobet A; Molecular Microbiology and Structural Biochemistry Laboratory (CNRS UMR 5086), University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France.
Robert X; Molecular Microbiology and Structural Biochemistry Laboratory (CNRS UMR 5086), University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France.
Falson P; Molecular Microbiology and Structural Biochemistry Laboratory (CNRS UMR 5086), University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France.
Chaptal V; Molecular Microbiology and Structural Biochemistry Laboratory (CNRS UMR 5086), University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France. Electronic address: vincent.chaptal@ibcp.fr.

Biochim Biophys Acta Biomembr ; 1863(11): 183693, 2021 11 01.

Article em En | MEDLINE | ID: mdl-34271006

Assuntos

Microscopia Crioeletrônica/métodos; Detergentes/química; Proteínas de Membrana/ultraestrutura; Polímeros/química; Solventes/química; Interações Hidrofóbicas e Hidrofílicas; Proteínas de Membrana/química; Conformação Proteica

Palavras-chave

Amphipols; Cryo-EM; Detergent belt; Membrane proteins; Nanodisc

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polímeros / Solventes / Microscopia Crioeletrônica / Detergentes / Proteínas de Membrana Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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