Structural and functional analysis of gum arabic l-rhamnose-&#945;-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family.

Kondo, Tatsuya; Kichijo, Miyu; Maruta, Akiho; Nakaya, Makoto; Takenaka, Shigeo; Arakawa, Takatoshi; Fushinobu, Shinya; Sakamoto, Tatsuji

Structural and functional analysis of gum arabic l-rhamnose-α-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family.

Kondo, Tatsuya; Kichijo, Miyu; Maruta, Akiho; Nakaya, Makoto; Takenaka, Shigeo; Arakawa, Takatoshi; Fushinobu, Shinya; Sakamoto, Tatsuji.

Afiliação

Kondo T; Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Kichijo M; Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Maruta A; Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Nakaya M; Center for Research and Development of Bioresources, Organization for Research Promotion, Osaka Prefecture University, Osaka, Japan; Department of Nutrition, Otemae College of Nutrition and Confectionery, Osaka, Japan.
Takenaka S; Graduate School of Comprehensive Rehabilitation, Osaka Prefecture University, Osaka, Japan.
Arakawa T; Department of Biotechnology, The University of Tokyo, Tokyo, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
Fushinobu S; Department of Biotechnology, The University of Tokyo, Tokyo, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
Sakamoto T; Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan. Electronic address: sakamoto@biochem.osakafu-u.ac.jp.

J Biol Chem ; 297(3): 101001, 2021 09.

Article em En | MEDLINE | ID: mdl-34303708

Assuntos

Ácido Glucurônico/metabolismo; Goma Arábica/metabolismo; Polissacarídeo-Liases/metabolismo; Ramnose/metabolismo; Sequência de Aminoácidos; Sequência de Bases; Cristalografia por Raios X; Fusarium/enzimologia; Filogenia; Polissacarídeo-Liases/química; Conformação Proteica; Proteínas Recombinantes/química; Proteínas Recombinantes/metabolismo; Relação Estrutura-Atividade; Especificidade por Substrato

Palavras-chave

Fusarium oxysporum; arabinogalactan protein; catalysis; gum arabic; l-rhamnose-α-1,4-d-glucuronate lyase; polysaccharide; polysaccharide lyase; structural biology; structurefunction; substrate specificity

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeo-Liases / Ramnose / Ácido Glucurônico / Goma Arábica Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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