Lignin valorization through efficient microbial production of ß-ketoadipate from industrial black liquor.
Bioresour Technol
; 337: 125489, 2021 Oct.
Article
em En
| MEDLINE
| ID: mdl-34320768
ABSTRACT
Vanillin and vanillate are the major lignin-derived aromatic compounds produced through the alkaline oxidation of softwood lignin. Because the production of higher-value added chemicals from these compounds is essential for lignin valorization, the microbial production of ß-ketoadipate, a promising raw material for the synthesis of novel nylons, from lignin was considered. Pseudomonas putida KT2440 was engineered to convert vanillin and vanillate to ß-ketoadipate. By examining the culture conditions with an initial culture volume of 1 L, the engineered strain completely converted 25 g of vanillin and 25 g of vanillate and produced approximately 23 g of ß-ketoadipate from each of them with a yield of 93% or higher. Furthermore, this strain showed the ability to efficiently produce ß-ketoadipate from softwood lignin extracts in black liquor, a byproduct of pulp production. These results suggest that the production of ß-ketoadipate from industrial black liquor is highly feasible for substantial lignin valorization.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas putida
/
Lignina
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article