Processing hair follicles for transmission electron microscopy.
Exp Dermatol
; 31(2): 110-121, 2022 02.
Article
em En
| MEDLINE
| ID: mdl-34351648
ABSTRACT
Transmission electron microscopy (TEM) has greatly advanced our knowledge of hair growth and follicle morphogenesis, but complex preparations such as fixation, dehydration and embedding compromise ultrastructure. While recent developments with cryofixation have been shown to preserve the ultrastructure of biological materials close to native state, they do have limitations. This review will focus on each stage of the TEM sample preparation process and their effects on the structural integrity of follicles.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Folículo Piloso
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article