Enzymatic Synthesis and Structural Confirmation of Novel Oligosaccharide, D-Fructofuranose-linked Chitin Oligosaccharide.
J Appl Glycosci (1999)
; 67(4): 129-135, 2020.
Article
em En
| MEDLINE
| ID: mdl-34354539
Utilizing transglycosylation reaction catalyzed by ß- N -acetylhexosaminidase of Stenotrophomonas maltophilia , ß-D-fructofuranosyl-(2â1)-α- N , N ´diacetylchitobioside (GlcNAc 2 -Fru) was synthesized from N -acetylsucrosamine and N , N ´-diacetylchitobiose (GlcNAc 2 ), and ß-D-fructofuranosyl-(2â1)-α- N , N ´, N ´´-triacetylchitotrioside (GlcNAc 3 -Fru) was synthesized from GlcNAc 2 -Fru and GlcNAc 2 . Through purification by charcoal column chromatography, pure GlcNAc 2 -Fru and GlcNAc 3 -Fru were obtained in molar yields of 33.0 % and 11.7 % from GlcNAc 2 , respectively. The structures of these oligosaccharides were confirmed by comparing instrumental analysis data of fragments obtained by enzymatic hydrolysis and acid hydrolysis of them with known data of these fragments.
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01-internacional
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MEDLINE
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En
Ano de publicação:
2020
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Article