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Differential Serotonin Uptake Mechanisms at the Human Maternal-Fetal Interface.
Bakovic, Petra; Kesic, Maja; Peric, Maja; Beceheli, Ivona; Horvaticek, Marina; George, Meekha; Cicin-Sain, Lipa; Desoye, Gernot; Wadsack, Christian; Panzenboeck, Ute; Stefulj, Jasminka.
Afiliação
  • Bakovic P; Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
  • Kesic M; Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
  • Peric M; Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
  • Beceheli I; Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
  • Horvaticek M; Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
  • George M; Otto Loewi Research Center, Division of Immunology and Pathophysiology, Medical University of Graz, Heinrichstrasse 31a, A-8010 Graz, Austria.
  • Cicin-Sain L; Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
  • Desoye G; Department of Obstetrics and Gynaecology, Medical University of Graz, Auenbruggerplatz 14, A-8036 Graz, Austria.
  • Wadsack C; Department of Obstetrics and Gynaecology, Medical University of Graz, Auenbruggerplatz 14, A-8036 Graz, Austria.
  • Panzenboeck U; Otto Loewi Research Center, Division of Immunology and Pathophysiology, Medical University of Graz, Heinrichstrasse 31a, A-8010 Graz, Austria.
  • Stefulj J; Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, HR-10000 Zagreb, Croatia.
Int J Mol Sci ; 22(15)2021 Jul 21.
Article em En | MEDLINE | ID: mdl-34360573
ABSTRACT
Serotonin (5-HT) plays an extensive role during pregnancy in regulating both the placental physiology and embryonic/fetal development. The uptake of 5-HT into cells is central to the control of local concentrations of 5-HT near its molecular targets. Here, we investigated the mechanisms of 5-HT uptake into human primary placental cells and cord blood platelets, all isolated immediately after birth. Trophoblasts and cord blood platelets showed 5-HT uptake with similar Michaelis constant (Km) values (~0.6 µM), typical of the high-affinity serotonin transporter (SERT). The uptake of 5-HT into trophoblasts was efficiently inhibited by various SERT-targeting drugs. In contrast, the uptake of 5-HT into feto-placental endothelial cells was not inhibited by a SERT blocker and showed a Km value (~782 µM) in the low-affinity range. Consistent with this, SERT mRNAs were abundant in term trophoblasts but sparse in feto-placental endothelial cells, whereas the opposite was found for the low-affinity plasma membrane monoamine transporter (PMAT) mRNAs. Organic cation transporter (OCT) 1, 2, and 3 mRNAs were absent or sparse in both cell types. In summary, the results demonstrate, for the first time, the presence of functional 5-HT uptake systems in feto-placental endothelial cells and fetal platelets, cells that are in direct contact with fetal blood plasma. The data also highlight the sensitivity to various psychotropic drugs of 5-HT transport into trophoblasts facing the maternal blood. The multiple, high-, and low-affinity systems present for the cellular uptake of 5-HT underscore the importance of 5-HT homeostasis at the maternal-fetal interface.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Placenta / Serotonina / Regulação da Expressão Gênica / Agonistas do Receptor de Serotonina / Proteínas da Membrana Plasmática de Transporte de Serotonina / Feto / Troca Materno-Fetal Limite: Female / Humans / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Placenta / Serotonina / Regulação da Expressão Gênica / Agonistas do Receptor de Serotonina / Proteínas da Membrana Plasmática de Transporte de Serotonina / Feto / Troca Materno-Fetal Limite: Female / Humans / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article