Triggering receptor expressed on myeloid cells-2 stimulates osteoclast differentiation and bone loss in periodontitis.
Oral Dis
; 28(6): 1652-1661, 2022 Sep.
Article
em En
| MEDLINE
| ID: mdl-34387017
ABSTRACT
OBJECTIVE:
To investigate the expression of triggering receptor expressed on myeloid cells 2 (TREM-2) in the healthy and diseased tissue, including gingivitis or periodontitis, and then to assess whether it has an impact on the development of periodontitis. METHODS AND MATERIALS The gingival tissues from healthy controls, gingivitis, and periodontitis underwent hematoxylin-eosin and immunohistochemical staining, and the association of TREM-2 expression or TREM-2+ cell counts with clinical parameters was assessed. An anti-TREM-2 antibody was used to block the osteoclastogenesis in vitro and during the experimental periodontitis by injection into the gingiva. The relative gene expression of TREM-2 in different gingival tissues was analyzed by quantitative PCR.RESULTS:
In the gingival tissues of periodontitis, TREM-2 expression and TREM-2+ cell counts were significantly higher than those of gingivitis and healthy controls (p<0.05). In the group of periodontitis showing moderate signs, the gingival tissues displayed significantly lower TREM-2 expression, in contrast with the group with advanced periodontal symptoms (p < 0.05). Consistently, blocking TREM-2 significantly decreased osteoclast formation both in vitro and in vivo (p < 0.05).CONCLUSION:
Increased TREM-2 expression and TREM-2+ cells were positively associated with the development of periodontitis. Osteoclast differentiation and stimulating alveolar bone loss were partly relied on TREM-2, which could be a target to be blocked for attenuating osteoclastogenesis in periodontitits.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Periodontite
/
Perda do Osso Alveolar
/
Gengivite
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article