A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae.
STAR Protoc
; 2(3): 100709, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34401776
ABSTRACT
Characterizing mRNA and protein expression with temporal and spatial resolution is a valuable component of nearly every developmental study. Here, we describe a protocol that combines in situ hybridization chain reaction (HCR) and immunofluorescence, allowing for the detection of mRNAs and proteins simultaneously, in zebrafish embryos and larvae. This protocol expands the flexibility of multiplexed HCR by coupling it with traditional immunofluorescence detection. For complete details on the use and execution of this protocol, please refer to Choi et al. (2010, 2016, 2018) and Howard et al. (2021).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hibridização in Situ Fluorescente
/
Hibridização In Situ
/
Perfilação da Expressão Gênica
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article