Your browser doesn't support javascript.
loading
miR-128 participates in the pathogenesis of chronic constipation by regulating the p38α/M-CSF inflammatory signaling pathway.
Hong, Yuntian; Ren, Xianghai; Liu, Weicheng; Sun, Kongliang; Chen, Baoxiang; Liu, Bo; Yu, Xueqiao; Chen, Quanjiao; Qian, Qun; Xie, Xiaoyu; Jiang, Congqing.
Afiliação
  • Hong Y; Department of Colorectal and Anal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
  • Ren X; Clinical Center of Intestinal and Colorectal Diseases of Hubei Province, Wuhan, People's Republic of China.
  • Liu W; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
  • Sun K; Department of Colorectal and Anal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
  • Chen B; Clinical Center of Intestinal and Colorectal Diseases of Hubei Province, Wuhan, People's Republic of China.
  • Liu B; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
  • Yu X; Colorectal and Anal Disease Research Center of Medical School, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
  • Chen Q; Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, People's Republic of China.
  • Qian Q; Department of Colorectal and Anal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
  • Xie X; Clinical Center of Intestinal and Colorectal Diseases of Hubei Province, Wuhan, People's Republic of China.
  • Jiang C; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
Am J Physiol Gastrointest Liver Physiol ; 321(4): G436-G447, 2021 10 01.
Article em En | MEDLINE | ID: mdl-34405716
ABSTRACT
Chronic constipation (CC) is a gastrointestinal disorder that adversely affects the quality of life. MicroRNAs are involved in the pathogenesis of functional gastrointestinal disorders. This study aims to investigate the molecular mechanism of microRNA-128 in CC. Here, we successfully constructed a murine model of CC based on morphine and rhubarb. The expression of stem cell factor (SCF) and neuron-specific enolase (NSE) was low in the models. Using miRNA array and bioinformatic analysis, we predicted and confirmed the expression of miR-128 and its downstream target genes in CC model. Compared with the control group, CC group showed a significant downregulation of miR-128 and upregulation of p38α and macrophage colony-stimulating factors (M-CSFs). Moreover, we observed elevated inflammatory cytokine and decreased anti-inflammatory cytokine levels in colonic tissues. Furthermore, coculture assays indicated that regulating expression of miR-128 in colonic epithelial cells induced the secretion of IL-6 and TNF-α by macrophages. In conclusion, our study demonstrated that miR-128 regulated the p38α/M-CSF signaling pathway to promote chronic inflammatory responses and changes in the immune microenvironment of the colon, thereby offering potential insights into the pathogenesis of CC and therapeutic targets for its treatment.NEW & NOTEWORTHY In this study, we constructed a murine model and identified a novel signaling mechanism involved in the chronic constipation progression. Our findings on the role of miR-128/p38α/M-CSF axis provide new insights into the treatment of chronic constipation.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Fator Estimulador de Colônias de Macrófagos / Constipação Intestinal / MicroRNAs / Proteínas Quinases p38 Ativadas por Mitógeno Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Fator Estimulador de Colônias de Macrófagos / Constipação Intestinal / MicroRNAs / Proteínas Quinases p38 Ativadas por Mitógeno Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article