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ZnT8 Deficiency Protects From APAP-Induced Acute Liver Injury by Reducing Oxidative Stress Through Upregulating Hepatic Zinc and Metallothioneins.
Su, Wen; Feng, Mingji; Liu, Yuan; Cao, Rong; Liu, Yiao; Tang, Junyao; Pan, Ke; Lan, Rongfeng; Mao, Zhuo.
Afiliação
  • Su W; School of Basic Medical Sciences, Shenzhen University Medical Center, Shenzhen University Health Science Center, Shenzhen, China.
  • Feng M; School of Basic Medical Sciences, Shenzhen University Medical Center, Shenzhen University Health Science Center, Shenzhen, China.
  • Liu Y; School of Basic Medical Sciences, Shenzhen University Medical Center, Shenzhen University Health Science Center, Shenzhen, China.
  • Cao R; Department of Nephrology, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
  • Liu Y; School of Basic Medical Sciences, Shenzhen University Medical Center, Shenzhen University Health Science Center, Shenzhen, China.
  • Tang J; School of Basic Medical Sciences, Shenzhen University Medical Center, Shenzhen University Health Science Center, Shenzhen, China.
  • Pan K; Institute for Advanced Study, Shenzhen University, Shenzhen, China.
  • Lan R; Department of Cell Biology and Medical Genetics, School of Basic Medical Sciences, Shenzhen University Health Science Center, Shenzhen, China.
  • Mao Z; School of Basic Medical Sciences, Shenzhen University Medical Center, Shenzhen University Health Science Center, Shenzhen, China.
Front Pharmacol ; 12: 721471, 2021.
Article em En | MEDLINE | ID: mdl-34413780
ABSTRACT
Zinc transporter 8 (ZnT8) is an important zinc transporter highly expressed in pancreatic islets. Deficiency of ZnT8 leads to a marked decrease in islet zinc, which is thought to prevent liver diseases associated with oxidative stress. Herein, we aimed to investigate whether loss of islet zinc affects the antioxidant capacity of the liver and acute drug-induced liver injury. To address this question, we treated ZnT8 knockout (KO) or wild-type control mice with 300 mg/ kg acetaminophen (APAP) or phosphate-buffered saline (PBS). Unexpectedly, we found that loss of ZnT8 in mice ameliorated APAP-induced injury and was accompanied by inhibition of c-Jun N-terminal kinase (JNK) activation, reduced hepatocyte death, and decreased serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). An increase in hepatic glutathione (GSH) was observed, corresponding to a decrease in malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) levels. APAP-induced inflammation and glycogen depletion were alleviated. In contrast, no significant changes were observed in cytochrome P450 family 2 subfamily E member 1 (CYP2E1), the main enzyme responsible for drug metabolism. Elevated levels of hepatic zinc and metallothionein (MT) were also observed, which may contribute to the hepatoprotective effect in ZnT8 KO mice. Taken together, these results suggest that ZnT8 deficiency protects the liver from APAP toxicity by attenuating oxidative stress and promoting hepatocyte proliferation. This study provides new insights into the functions of ZnT8 and zinc as key mediators linking pancreatic and hepatic functions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article