Application of l-Threonine Aldolase to on-DNA Reactions.
Bioconjug Chem
; 32(9): 1973-1978, 2021 09 15.
Article
em En
| MEDLINE
| ID: mdl-34424686
ABSTRACT
Enzymatic catalysis is a highly attractive approach to the DNA encoded library technology (DEL) that has not been widely explored. In this paper, we report an l-threonine aldolase (l-TA)-catalyzed on-DNA aldol reaction to form ß-hydroxy-α-amino acids, and its diastereoselectivity determination. l-TAs from three species show good on-DNA aldehyde scope and complementary stereoselectivity. The formed aldol product can be further diversified via various reactions, which demonstrates the utility of this reaction in DEL.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicina Hidroximetiltransferase
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article