In utero intraocular AAV injection for early gene expression in the developing rodent retina.
STAR Protoc
; 2(3): 100742, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34430915
ABSTRACT
The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the expression of transgenes early in the developing retina are essential to study visual system development. Here, we describe a protocol to express genes-of-interest in the developing mouse retina via in utero intraocular adeno-associated virus injections. For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2021).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Transgenes
/
Injeções Intraoculares
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article