Discovery and validation of peritoneal endometriosis biomarkers in peritoneal fluid and serum.
Reprod Biomed Online
; 43(4): 727-737, 2021 Oct.
Article
em En
| MEDLINE
| ID: mdl-34446375
ABSTRACT
RESEARCH QUESTION What are the potential biomarkers for peritoneal endometriosis in peritoneal fluid and serum? DESIGN:
Case-control studies composed of independent discovery and validation sets were conducted. In the discovery set, untargeted liquid chromatography-mass spectrometry (LC-MS/MS) metabolomics, multivariable and univariable analyses were conducted to generate global metabolomic profiles of peritoneal fluid for endometriosis and to identify potential metabolites that could distinguish peritoneal endometriosis (nâ¯=â¯10) from controls (nâ¯=â¯31). The identified metabolites from the discovery set were validated in independent peritoneal fluid (n =19 peritoneal endometriosis and nâ¯=â¯20 controls) and serum samples (nâ¯=â¯16 peritoneal endometriosis and nâ¯=â¯19 controls) using targeted metabolomics. The area under the receiver-operating characteristics curve (AUC) analysis was used to evaluate the diagnostic performance of peritoneal endometriosis metabolites.RESULTS:
In the discovery set, peritoneal fluid phosphatidylcholine (343) and phenylalanyl-isoleucine were significantly increased in peritoneal endometriosis groups compared with control groups, with AUC 0.77 (95% CI 0.61 to 0.92; Pâ¯=â¯0.018) and AUC 0.98 (95% CI 0.95 to 1.02; P < 0.001), respectively. In the validation set, phenylalanyl-isoleucine retained discriminatory performance to distinguish peritoneal endometriosis from controls in both peritoneal fluid (AUC 0.77, 95% CI 0.61 to 0.92; Pâ¯=â¯0.006) and serum samples (AUC 0.81, 95% CI 0.64 to 0.99; Pâ¯=â¯0.004), with notably stronger discrimination between peritoneal endometriosis and controls in proliferative phase.CONCLUSION:
Our preliminary results propose phenylalanyl-isoleucine as a potential biomarker of peritoneal endometriosis, which may be used as a minimally invasive diagnostic biomarker of peritoneal endometriosis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Peritoneais
/
Líquido Ascítico
/
Endometriose
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Adult
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article