Large-Scale Transient Production in ExpiCHO-S&#8482; with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection.

Zhong, Xiaotian; Schwab, Ashley; Ma, Weijun; Meade, Caryl L; Zhou, Jing; D'Antona, Aaron M; Somers, Will; Lin, Laura

Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection.

Zhong, Xiaotian; Schwab, Ashley; Ma, Weijun; Meade, Caryl L; Zhou, Jing; D'Antona, Aaron M; Somers, Will; Lin, Laura.

Afiliação

Zhong X; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA. xiaotian.zhong@pfizer.com.
Schwab A; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
Ma W; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
Meade CL; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
Zhou J; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
D'Antona AM; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
Somers W; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
Lin L; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.

Methods Mol Biol ; 2313: 143-150, 2022.

Article em En | MEDLINE | ID: mdl-34478135

Assuntos

Transfecção; Animais; Células CHO; Cricetinae; Cricetulus; Processamento de Proteína Pós-Traducional; Proteínas Recombinantes

Palavras-chave

Chinese Hamster Ovary (CHO) Cells; N-linked glycosylation and sialylation; Polyethylenimine; Therapeutic glycoproteins; Transfection; Transient expression

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transfecção Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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