Mutually Orthogonal Bioconjugation of Vinyl Nucleosides for RNA Metabolic Labeling.
Org Lett
; 23(18): 7183-7187, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34496205
ABSTRACT
We report a strategy for the orthogonal conjugation of the vinyl nucleosides, 5-vinyluridine (5-VU) and 2-vinyladenosine (2-VA), via selective reactivity with maleimide and tris(2-carboxyethyl)phosphine (TCEP), respectively. The orthogonality was investigated using density functional theory (DFT) and confirmed by reactions with vinyl nucleosides. Further, these chemistries were used to modify RNA for fluorescent cell imaging. These reactions allow for the expanded use of RNA metabolic labeling to study nascent RNA expression within different RNA populations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfinas
/
RNA
/
Maleimidas
/
Nucleosídeos
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article