A Mutation in DNA Polymerase α Rescues WEE1KO Sensitivity to HU.
Int J Mol Sci
; 22(17)2021 Aug 30.
Article
em En
| MEDLINE
| ID: mdl-34502313
ABSTRACT
During DNA replication, the WEE1 kinase is responsible for safeguarding genomic integrity by phosphorylating and thus inhibiting cyclin-dependent kinases (CDKs), which are the driving force of the cell cycle. Consequentially, wee1 mutant plants fail to respond properly to problems arising during DNA replication and are hypersensitive to replication stress. Here, we report the identification of the polα-2 mutant, mutated in the catalytic subunit of DNA polymerase α, as a suppressor mutant of wee1. The mutated protein appears to be less stable, causing a loss of interaction with its subunits and resulting in a prolonged S-phase.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência a Medicamentos
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Proteínas Serina-Treonina Quinases
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Arabidopsis
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Proteínas de Arabidopsis
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DNA Polimerase I
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Hidroxiureia
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Mutação
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article