Mitokyne: A Ratiometric Raman Probe for Mitochondrial pH.
Anal Chem
; 93(37): 12786-12792, 2021 09 21.
Article
em En
| MEDLINE
| ID: mdl-34505518
ABSTRACT
Mitochondrial pH (pHmito) is intimately related to mitochondrial function, and aberrant values for pHmito are linked to several disease states. We report the design, synthesis, and application of mitokyne 1-the first small molecule pHmito sensor for stimulated Raman scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pHmito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mitofagia
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article