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Heterologous expression of a single fungal HR-PKS leads to the formation of diverse 2-alkenyl-tetrahydropyrans in model fungi.
Lyu, Hai-Ning; Zhang, Jinyu; Zhou, Shuang; Liu, Hong-Wei; Zhuang, Wen-Ying; Li, Shu-Ming; Yin, Wen-Bing.
Afiliação
  • Lyu HN; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. yinwb@im.ac.cn.
  • Zhang J; Artemisinin Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, People's Republic of China.
  • Zhou S; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. yinwb@im.ac.cn.
  • Liu HW; Savaid Medical School, University of Chinese Academy of Sciences, Beijing100049, People's Republic of China.
  • Zhuang WY; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. yinwb@im.ac.cn.
  • Li SM; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. yinwb@im.ac.cn.
  • Yin WB; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. yinwb@im.ac.cn.
Org Biomol Chem ; 19(38): 8377-8383, 2021 10 06.
Article em En | MEDLINE | ID: mdl-34528986
2-Alkenyl-tetrahydropyrans belong to a rare class of natural products that exhibit broad antifungal activities. Their structural instability and rareness in nature have restrained their discovery and drug development. In this study, the heterologous expression of a single highly reducing polyketide synthase (HR-PKS, App1) from Trichoderma applanatum in Aspergillus nidulans leads to the formation of seven 2-alkenyl-tetrahydropyran derivatives including one known compound virensol C (1) and six new compounds (2-7). However, introducing App1 into Saccharomyces cerevisiae resulted in the identification of additional two 2-alkenyl-tetrahydropyrans lacking the hydroxyl or methoxyl group at the C-2 position (8 and 9). The structures of the isolated compounds were elucidated by extensive spectroscopic analysis using NMR and HR-ESI-MS.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Policetídeo Sintases Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Policetídeo Sintases Idioma: En Ano de publicação: 2021 Tipo de documento: Article