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Upregulation of α enolase (ENO1) crotonylation in colorectal cancer and its promoting effect on cancer cell metastasis.
Hou, Jia-Yi; Cao, Jing; Gao, Li-Juan; Zhang, Fu-Peng; Shen, Jing; Zhou, Lan; Shi, Jian-Yun; Feng, Yan-Lin; Yan, Zi; Wang, De-Ping; Cao, Ji-Min.
Afiliação
  • Hou JY; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China; Department of Clinical Laboratory, Shanxi Provincial Academy of Traditiona
  • Cao J; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Gao LJ; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Zhang FP; National Clinical Research Base of Traditional Chinese Medicine, Shanxi Hospital of Traditional Chinese Medicine, Taiyuan, China.
  • Shen J; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Zhou L; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Shi JY; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Feng YL; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Yan Z; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Wang DP; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China.
  • Cao JM; Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, And the Department of Physiology, Shanxi Medical University, Taiyuan, China. Electronic address: caojimin@sxmu.edu.cn.
Biochem Biophys Res Commun ; 578: 77-83, 2021 11 12.
Article em En | MEDLINE | ID: mdl-34547627
ABSTRACT
Lysine crotonylation (Kcr) is a newly identified protein translational modification and is involved in major biological processes including glycolysis, but its role in colorectal cancer (CRC) is unknown. Here, we found that the Kcr of α enolase (ENO1) was significantly elevated in human CRC tissues compared with the paratumoral tissues. CREB-binding protein (CBP) functioned as a crotonyltranferase of ENO1, and SIRT2 was involved in the decrotonylation of ENO1. Using quantitative mass spectrometry for crotonylomics analysis, we further found that K420 was the main Kcr site of ENO1 and ENO1 K420 Kcr promoted the growth, migration, and invasion of CRC cells in vitro by enhancing the activity of ENO1 and regulating the expression of tumor-associated genes. Our study reveals an important mechanism by which ENO1 regulates CRC through crotonylation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfopiruvato Hidratase / Neoplasias Colorretais / Biomarcadores Tumorais / Processamento de Proteína Pós-Traducional / Proteínas Supressoras de Tumor / Proteínas de Ligação a DNA / Proteína de Ligação a CREB / Sirtuína 2 / Lisina Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfopiruvato Hidratase / Neoplasias Colorretais / Biomarcadores Tumorais / Processamento de Proteína Pós-Traducional / Proteínas Supressoras de Tumor / Proteínas de Ligação a DNA / Proteína de Ligação a CREB / Sirtuína 2 / Lisina Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article