Upregulation of α enolase (ENO1) crotonylation in colorectal cancer and its promoting effect on cancer cell metastasis.
Biochem Biophys Res Commun
; 578: 77-83, 2021 11 12.
Article
em En
| MEDLINE
| ID: mdl-34547627
ABSTRACT
Lysine crotonylation (Kcr) is a newly identified protein translational modification and is involved in major biological processes including glycolysis, but its role in colorectal cancer (CRC) is unknown. Here, we found that the Kcr of α enolase (ENO1) was significantly elevated in human CRC tissues compared with the paratumoral tissues. CREB-binding protein (CBP) functioned as a crotonyltranferase of ENO1, and SIRT2 was involved in the decrotonylation of ENO1. Using quantitative mass spectrometry for crotonylomics analysis, we further found that K420 was the main Kcr site of ENO1 and ENO1 K420 Kcr promoted the growth, migration, and invasion of CRC cells in vitro by enhancing the activity of ENO1 and regulating the expression of tumor-associated genes. Our study reveals an important mechanism by which ENO1 regulates CRC through crotonylation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfopiruvato Hidratase
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Neoplasias Colorretais
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Biomarcadores Tumorais
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Processamento de Proteína Pós-Traducional
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Proteínas Supressoras de Tumor
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Proteínas de Ligação a DNA
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Proteína de Ligação a CREB
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Sirtuína 2
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Lisina
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article