Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.
STAR Protoc
; 2(4): 100842, 2021 12 17.
Article
em En
| MEDLINE
| ID: mdl-34585169
ABSTRACT
Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Células Mieloides
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article