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An adaptive optics module for deep tissue multiphoton imaging in vivo.
Rodríguez, Cristina; Chen, Anderson; Rivera, José A; Mohr, Manuel A; Liang, Yajie; Natan, Ryan G; Sun, Wenzhi; Milkie, Daniel E; Bifano, Thomas G; Chen, Xiaoke; Ji, Na.
Afiliação
  • Rodríguez C; Department of Physics, University of California, Berkeley, CA, USA.
  • Chen A; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Rivera JA; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
  • Mohr MA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Liang Y; Bio Optical and Acoustic Spectroscopy Laboratory, Neurophotonics Center, Boston University, Boston, MA, USA.
  • Natan RG; Department of Physics, University of California, Berkeley, CA, USA.
  • Sun W; Department of Biology, Stanford University, Stanford, CA, USA.
  • Milkie DE; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Bifano TG; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
  • Chen X; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Ji N; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Nat Methods ; 18(10): 1259-1264, 2021 10.
Article em En | MEDLINE | ID: mdl-34608309
ABSTRACT
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óptica e Fotônica / Neuroimagem Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óptica e Fotônica / Neuroimagem Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article