The interplay of protein engineering and glycoengineering to fine-tune antibody glycosylation and its impact on effector functions.

Wang, Qiong; Wang, Tiexin; Zhang, Roushu; Yang, Shuang; McFarland, Kevin S; Chung, Cheng-Yu; Jia, Hongpeng; Wang, Lai-Xi; Cipollo, John F; Betenbaugh, Michael J

Wang, Qiong; Wang, Tiexin; Zhang, Roushu; Yang, Shuang; McFarland, Kevin S; Chung, Cheng-Yu; Jia, Hongpeng; Wang, Lai-Xi; Cipollo, John F; Betenbaugh, Michael J.

Afiliação

Wang Q; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Wang T; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Zhang R; Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, USA.
Yang S; Division of Bacterial, Parasitic and Allergenic Products (DBPAP), Laboratory for Bacterial Polysaccharides, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
McFarland KS; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Chung CY; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Jia H; Division of Pediatric Surgery, Department of Surgery, Johns Hopkins University, Baltimore, Maryland, USA.
Wang LX; Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, USA.
Cipollo JF; Division of Bacterial, Parasitic and Allergenic Products (DBPAP), Laboratory for Bacterial Polysaccharides, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
Betenbaugh MJ; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.

Biotechnol Bioeng ; 119(1): 102-117, 2022 01.

Article em En | MEDLINE | ID: mdl-34647616

Assuntos

Imunoglobulina G; Polissacarídeos; Engenharia de Proteínas/métodos; Animais; Citotoxicidade Celular Dependente de Anticorpos/genética; Células CHO; Cricetinae; Cricetulus; Fucose/química; Fucose/metabolismo; Glicosilação; Fragmentos Fc das Imunoglobulinas/química; Fragmentos Fc das Imunoglobulinas/genética; Fragmentos Fc das Imunoglobulinas/imunologia; Imunoglobulina G/química; Imunoglobulina G/genética; Imunoglobulina G/imunologia; Mutação/genética; Ácido N-Acetilneuramínico/química; Ácido N-Acetilneuramínico/metabolismo; Polissacarídeos/química; Polissacarídeos/metabolismo; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/imunologia

Palavras-chave

Antibody glycosylation; Core fucose; Effector functions; Fc mutation; Sialylation linkage

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos / Imunoglobulina G / Engenharia de Proteínas Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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