Extracellular vesicles from tonsilderived mesenchymal stromal cells show antitumor effect via miR199a3p.
Int J Mol Med
; 48(6)2021 12.
Article
em En
| MEDLINE
| ID: mdl-34676871
ABSTRACT
Mesenchymal stem cells (MSCs) are mesodermoriginated adult SCs that possess multidirectional differentiation potential. MSCs migrate to injured tissue and secrete a range of paracrine factors that induce regeneration in damaged tissue and exert immune modulation. Because tumor progression is dependent on crosstalk between the tumor and its microenvironment, MSCs also produce extracellular vesicles (EVs) that mediate information transfer in the tumor microenvironment. However, the effect of MSCderived EVs on tumor development and progression is still controversial. To date, tonsilderived MSCs (TMSCs) have been shown to possess all the defined characteristics of MSCs and show distinctive features of differential potential and immune modulation. To observe the effect of soluble mediators from TMSCs on tumor growth, human liver cancer cell line (HepG2) cells were injected into nude mice and HepG2 cell scratch migration assay was performed using conditioned medium (CM) of TMSCs. TMSC CM inhibited tumor growth and progression and it was hypothesized that EVs from TMSCs could inhibit tumor progression. microRNA (miRNA or miR) sequencing using five different origins of TMSCderived EVs was performed and highly expressed miRNAs, such as miR199a3p, miR2143p, miR199a5p and miR199b5p, were selected. TMSCs inhibited tumor growth and HepG2 cell migration, potentially via miR199a3p targeting CD151, integrin α3 and 6 in HepG2 cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tonsila Palatina
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MicroRNAs
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Células-Tronco Mesenquimais
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Vesículas Extracelulares
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Neoplasias Hepáticas
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article