An efficient single-cell based method for linking human T cell phenotype to T cell receptor sequence and specificity.
Eur J Immunol
; 52(2): 237-246, 2022 02.
Article
em En
| MEDLINE
| ID: mdl-34710239
ABSTRACT
Single-cell antigen-receptor gene amplification and sequencing platforms have been used to characterize T cell receptor (TCR) repertoires but typically fail to generate paired full-length gene products for direct expression cloning and do not enable linking this data to cell phenotype information. To overcome these limitations, we established a high-throughput platform for the quantitative and qualitative analysis of human TCR repertoires that provides insights into the clonal and functional composition of human CD4+ and CD8+ αß T cells at the molecular and cellular level. The strategy is a powerful tool to qualitatively assess differences between antigen receptors of phenotypically defined αß T cell subsets, e.g. in immune responses to cancer, vaccination, or infection, and in autoimmune diseases.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T CD4-Positivos
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Receptores de Antígenos de Linfócitos T alfa-beta
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Linfócitos T CD8-Positivos
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Análise de Célula Única
Tipo de estudo:
Qualitative_research
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article