Quorum quenching affects biofilm development in an anaerobic membrane bioreactor (AnMBR): from macro to micro perspective.

ABSTRACT

The first experimental study on the influence of acyl homoserine lactones (AHLs) degrading quorum quenching (QQ) consortium on the dynamics of biofilm bio-communities (i.e., from suspended biomass to initial biofilm and mature biofilm) in an anaerobic membrane bioreactor (AnMBR) at a microscopic scale (denoted as QQAnMBR) was reported. QQ did not change the overall bacterial community of the suspended biomass, inclusive of the key functional bacteria. Moreover, the retarded initial biofilm formation was attributed to not only the lower extracellular polymeric substance content of suspended biomass, but also the decelerated colonization of the AHL-regulated low-abundance in suspended biomass but pioneering keystone taxa Rhodocyclaceae;g- on membrane surface. However, pioneering fouling-related taxa such as Sulfurovum and Rhodocyclaceae;g- still played paramount roles in the delayed initial biofilm formation in the QQAnMBR. Furthermore, the microbial assemblies of the mature biofilm were changed in the QQAnMBR, probably attributable to the abiotic microbial floc attachment.