Gold-based immunochromatographic assay strip for the detection of quinclorac in foods.
Analyst
; 146(22): 6831-6839, 2021 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-34723310
ABSTRACT
In this study, a highly specific and sensitive monoclonal antibody (mAb) against quinclorac (Qui) was prepared. Based on the selected mAb, 2G3, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip assay were established for the detection of Qui in actual samples. The 50%-inhibitory concentration of mAb 2G3 against Qui was 48.763 ng mL-1. No cross-reaction with other quinolines indicated that mAb 2G3 had high specificity. The recovery of the established ic-ELISA method was in the range of 85.6% to 98.9%. The cut-off value of Qui in cucumber and tomato by immunochromatographic strip was 200 ng g-1. The analysis results of ic-ELISA and immunochromatographic strip assay were consistent with the results of LC-MS/MS, which further proved that the established ic-ELISA and immunochromatographic strip assay could provide valuable tools for the rapid detection of Qui residues in cucumber and tomato samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinolinas
/
Ouro
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article