Challenges and approaches to studying pore-forming proteins.
Biochem Soc Trans
; 49(6): 2749-2765, 2021 12 17.
Article
em En
| MEDLINE
| ID: mdl-34747994
ABSTRACT
Pore-forming proteins (PFPs) are a broad class of molecules that comprise various families, structural folds, and assembly pathways. In nature, PFPs are most often deployed by their host organisms to defend against other organisms. In humans, this is apparent in the immune system, where several immune effectors possess pore-forming activity. Furthermore, applications of PFPs are found in next-generation low-cost DNA sequencing, agricultural crop protection, pest control, and biosensing. The advent of cryoEM has propelled the field forward. Nevertheless, significant challenges and knowledge-gaps remain. Overcoming these challenges is particularly important for the development of custom, purpose-engineered PFPs with novel or desired properties. Emerging single-molecule techniques and methods are helping to address these unanswered questions. Here we review the current challenges, problems, and approaches to studying PFPs.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Porinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article