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Equipment-free detection of SARS-CoV-2 and Variants of Concern using Cas13.
Arizti-Sanz, Jon; Bradley, A'Doriann; Zhang, Yibin B; Boehm, Chloe K; Freije, Catherine A; Grunberg, Michelle E; Kosoko-Thoroddsen, Tinna-Solveig F; Welch, Nicole L; Pillai, Priya P; Mantena, Sreekar; Kim, Gaeun; Uwanibe, Jessica N; John, Oluwagboadurami G; Eromon, Philomena E; Kocher, Gregory; Gross, Robin; Lee, Justin S; Hensley, Lisa E; Happi, Christian T; Johnson, Jeremy; Sabeti, Pardis C; Myhrvold, Cameron.
Afiliação
  • Arizti-Sanz J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Bradley A; Harvard-MIT Program in Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
  • Zhang YB; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Boehm CK; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Freije CA; Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA.
  • Grunberg ME; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Kosoko-Thoroddsen TF; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
  • Welch NL; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Pillai PP; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Mantena S; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
  • Kim G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Uwanibe JN; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • John OG; Program in Virology, Harvard Medical School, Boston, MA 02115, USA.
  • Eromon PE; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Kocher G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Gross R; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
  • Lee JS; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
  • Hensley LE; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.
  • Happi CT; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria.
  • Johnson J; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria.
  • Sabeti PC; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.
  • Myhrvold C; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and infectious diseases, National Institute of Health, Frederick, MD 21702, USA.
medRxiv ; 2021 Nov 02.
Article em En | MEDLINE | ID: mdl-34751276
ABSTRACT
The COVID-19 pandemic, and the recent rise and widespread transmission of SARS-CoV-2 Variants of Concern (VOCs), have demonstrated the need for ubiquitous nucleic acid testing outside of centralized clinical laboratories. Here, we develop SHINEv2, a Cas13-based nucleic acid diagnostic that combines quick and ambient temperature sample processing and lyophilized reagents to greatly simplify the test procedure and assay distribution. We benchmarked a SHINEv2 assay for SARS-CoV-2 detection against state-of-the-art antigen-capture tests using 96 patient samples, demonstrating 50-fold greater sensitivity and 100% specificity. We designed SHINEv2 assays for discriminating the Alpha, Beta, Gamma and Delta VOCs, which can be read out visually using lateral flow technology. We further demonstrate that our assays can be performed without any equipment in less than 90 minutes. SHINEv2 represents an important advance towards rapid nucleic acid tests that can be performed in any location.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article