A low-cost and open-source protocol to produce key enzymes for molecular detection assays.
STAR Protoc
; 2(4): 100899, 2021 12 17.
Article
em En
| MEDLINE
| ID: mdl-34766029
ABSTRACT
Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- and middle-income countries. The enzymes produced with this protocol have been successfully used for molecular detection applications. For complete details on the use and execution of this protocol, please refer to Alcántara et al. (2021a, 2021b).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Enzimas
/
Escherichia coli
Tipo de estudo:
Diagnostic_studies
/
Health_economic_evaluation
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article