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The draft chromosome-level genome assembly of tetraploid ground cherry (Prunus fruticosa Pall.) from long reads.
Wöhner, Thomas W; Emeriewen, Ofere F; Wittenberg, Alexander H J; Schneiders, Harrie; Vrijenhoek, Ilse; Halász, Júlia; Hrotkó, Károly; Hoff, Katharina J; Gabriel, Lars; Lempe, Janne; Keilwagen, Jens; Berner, Thomas; Schuster, Mirko; Peil, Andreas; Wünsche, Jens; Kropop, Stephan; Flachowsky, Henryk.
Afiliação
  • Wöhner TW; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany. Electronic address: thomas.woehner@julius-kuehn.de.
  • Emeriewen OF; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany.
  • Wittenberg AHJ; Keygene N.V., P.O. Box 216, 6700 AE Wageningen, Netherlands.
  • Schneiders H; Keygene N.V., P.O. Box 216, 6700 AE Wageningen, Netherlands.
  • Vrijenhoek I; Keygene N.V., P.O. Box 216, 6700 AE Wageningen, Netherlands.
  • Halász J; Department of Genetics and Plant Breeding, Faculty of Horticultural Science, Szent István University, Ménesi Str. 44, Budapest 1118, Hungary.
  • Hrotkó K; Department of Floriculture and Dendrology, Institute of Landscape Architecture, Urban Planning and Ornamental Horticulture, Hungarian University of Agriculture and Life Science, Villányi Str. 35-43, Budapest 1118, Hungary.
  • Hoff KJ; Institute of Mathematics and Computer Science, University of Greifswald, Walther-Rathenau-Str. 47, 17489 Greifswald, Germany; Center for Functional Genomics of Microbes, University of Greifswald, Felix-Hausdorff-Str. 8, 17489 Greifswald, Germany.
  • Gabriel L; Institute of Mathematics and Computer Science, University of Greifswald, Walther-Rathenau-Str. 47, 17489 Greifswald, Germany; Center for Functional Genomics of Microbes, University of Greifswald, Felix-Hausdorff-Str. 8, 17489 Greifswald, Germany.
  • Lempe J; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany.
  • Keilwagen J; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biosafety in Plant Biotechnology, Erwin-Baur-Str. 27, D-06484 Quedlinburg, Germany.
  • Berner T; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biosafety in Plant Biotechnology, Erwin-Baur-Str. 27, D-06484 Quedlinburg, Germany.
  • Schuster M; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany.
  • Peil A; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany.
  • Wünsche J; University of Hohenheim, Institute of Special Crops and Crop Physiology, 70593 Stuttgart, Germany.
  • Kropop S; we.heart.codes, Jagdweg 1-3, 01159 Dresden, Germany.
  • Flachowsky H; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany.
Genomics ; 113(6): 4173-4183, 2021 11.
Article em En | MEDLINE | ID: mdl-34774678
ABSTRACT
Cherries are stone fruits and belong to the economically important plant family of Rosaceae with worldwide cultivation of different species. The ground cherry, Prunus fruticosa Pall., is an ancestor of cultivated sour cherry, an important tetraploid cherry species. Here, we present a long read chromosome-level draft genome assembly and related plastid sequences using the Oxford Nanopore Technology PromethION platform and R10.3 pore type. We generated a final consensus genome sequence of 366 Mb comprising eight chromosomes. The N50 scaffold was ~44 Mb with the longest chromosome being 66.5 Mb. The chloroplast and mitochondrial genomes were 158,217 bp and 383,281 bp long, which is in accordance with previously published plastid sequences. This is the first report of the genome of ground cherry (P. fruticosa) sequenced by long read technology only. The datasets obtained from this study provide a foundation for future breeding, molecular and evolutionary analysis in Prunus studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prunus / Physalis Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prunus / Physalis Idioma: En Ano de publicação: 2021 Tipo de documento: Article