Study on the molecular mechanism of Laccaria bicolor helping Populus trichocarpa to resist the infection of Botryosphaeria dothidea.

AIMS: This study explored the specific molecular mechanism of Laccaria bicolor to help Populus trichocarpa resist infection by Botryosphaeria dothidea. METHODS AND RESULTS: Transcriptome technology was used to sequence P. trichocarpa under disease stress, and a total of 6379 differentially expressed genes (DEGs) were identified. A total of 536 new DEGs were induced by L. bicolor during the infection of B. dothidea. L. bicolor helps to prevent and alleviate the infection of B. dothidea by regulating related genes in the cell wall pathway, signal transduction pathway, disease-resistant protein synthesis pathway and antioxidant enzyme synthesis pathway of P. trichocarpa. CONCLUSION: The inoculation of L. bicolor can regulate the expression of genes in the cell wall pathway and enhance the physical defense capabilities of plants. Under disease stress conditions, L. bicolor can regulate signal transduction pathways, disease-resistant related pathways and reactive oxygen species (ROS) clearance pathways to help P. trichocarpa alleviate the disease. SIGNIFICANCE AND IMPACT OF THE STUDY: The research reveals the mechanism of L. bicolor inducing resistance to canker of P. trichocarpa from the molecular level and provides a theoretical basis for the practical application of mycorrhizal fungi to improve plant disease resistance.